Baculovirus DNA Cloning & Preparation

Clone the gene of interest into the suitable baculovirus transfer vector.

($1500/ one week)
-PCR/restriction enzyme digestion will be used to clone the gene of interest into a baculovirus transfer vector.
-If necessary, the subclone vector will be confirmed using restriction enzyme digestion and DNA sequence.
-The recombinant transfer vector will be prepared for further experiment. 

Generate recombinant baculovirus for target gene

($2500/ three weeks)
-Transfect the insect sf9 cells with recombinant transfer vector and baculovirus DNA.
-The recombinant virus will be screened by plaque assay.
-The recombinant virus will be amplified to a 30mL high-titer stock.

Amplification of recombinant baculovirus for titer stock. ($2500/ two weeks)
-A 250mL high-titer virus stock will be generated from a low-titer stock of a recombinant virus.
-Several rounds of amplification may be required in order to increase the recombinant virus titer stock.

Recombinant Protein Expression in sf9 cells

($2000/ one week)  
-One liter of insect cell culture will be generated.
-Cell pellet will be harvested and delivered.

Recombinant Protein Purification*

($2000/ one week)
-The protein will be purified using affinity column of His-tag and GST-tag recombinant proteins.

1. Purification development is not included and protein yield is variable under the baculovirus vector expression system.
2. We offer and provide optimization for protein expression, which is based on M.O.I. and different cell lines. Optimization is optional.

3. Please call us at (619) 788-8188 for a free consultation or email us at info@sheatechinc.com for additional information